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Stem Cell Research

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DEF-CS Culture System for Expanding Human Induced Pluripotent Stem Cells

The DEF-CS system is a robust culture system for efficient expansion of human induced pluripotent stem (iPS) cells in a feeder-free and defined environment. This system enables a stable growth rate that is equally ideal for traditional iPS cell culture, mass production of cells, and single cell culture. Cells grown with this culture system are maintained in an undifferentiated state with virtually no detectable background differentiation, eliminating the need for cell selection. Enzymatic passaging as single cells allows for single-cell applications, including high-throughput screening, transfection, and scaffold seeding.

The DEF-CS kit is a complete system that includes basal medium, additives, and a coating substrate. This system is compatible with human iPS cells.

  At-A-Glance   Documents   Images & Data   Resources


  • Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
  • Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal and differentiation
  • Complete kit includes 500 ml basal medium, additives, and coating compound
  • Robust system provides high reproducibility and stable growth
  • Maintains cells in an undifferentiated state with virtually no background differentiation


  • Scale-up and mass production of human iPS cells
  • Single-cell culture of human iPS cells
  • Transfection and reprogramming
  • High-throughput screening
  • Tissue engineering (seeding cells on a scaffold)


Published Examples of Use

  1. Boreström, C. et al. (2014) Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: A first step toward a clinical-grade cell source. Stem Cells Trans. Med. 3:433–447.
  2. Hanson, C. et al. (2013) Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro. Acta ophthalmologica 91(2):127–130.
  3. Kia, R. et al. (2014) MicroRNA-122: a novel hepatocyte-enriched in vitro marker of drug-induced cellular toxicity. Toxicological Sciences kfu269.
  4. Norrman, K. et al. (2013) Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level. Methods 59(1):59–70.
  5. Sivertsson, L. et al. (2012) Hepatic differentiation and maturation of human embryonic stem cells cultured in a perfused three-dimensional bioreactor.  Stem cells and development 22(4):581–594.
  6. Ulvestad, M. et al. (2013) Drug metabolizing enzyme and transporter protein profiles of hepatocytes derived from human embryonic and induced pluripotent stem cells. Biochemical pharmacology 86(5):691–702.
  7. Valton, J. et al. (2014) Efficient strategies for TALEN-mediated genome editing in mammalian cell lines. Methods 69(2):151–170.
  8. Zandén, C. et al. (2014) Stem cell responses to plasma surface modified electrospun polyurethane scaffolds. Nanomedicine: Nanotechnology, Biology and Medicine 10(5):949–958.

Additional Information

Please refer to technical documents under the Documents tab for storage conditions, product components, and technical specifications.

Cat. # Product Contents Size Price License Units Select
Y30012 Cellartis® DEF-CS™ 500 COAT-1 4 mL $232.00   *
Y30010 Cellartis® DEF-CS™ 500 Culture System 1 Kit $495.00 License Statements *
Y30017 Cellartis® DEF-CS™ 500 Basal Medium with Additives 500 mL $397.00 License Statements *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact



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